Record Details
Kinetic analysis of the aerobic degradation of chlorinated ethenes by the mycobacterium ELW-1 and chlorinated ethanes and ethenes by rhodococcus rhodochrous ATCC® 21198™
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Kinetic analysis of the aerobic degradation of chlorinated ethenes by the mycobacterium ELW-1 and chlorinated ethanes and ethenes by rhodococcus rhodochrous ATCC® 21198™ |
| Names |
Rich, Stephanie L.
(creator) Semprini, Lewis (advisor) |
| Date Issued | 2015-05-19 (iso8601) |
| Note | Honors Bachelor of Science (HBS) |
| Abstract | Bioremediation of chlorinated ethenes by the augmentation of contaminated soils with microorganisms is a proposed method of reducing contaminant concentrations in groundwater. To determine the viability of using ELW-1 or R. rhodochrous for groundwater remediation, kinetic analyses of transformation rates must be performed. The focus of this research was to determine: 1) the specific rates of contaminant transformation when ELW-1 was exposed to chlorinated ethenes, 2) if R. rhodochrous has the ability to transform chlorinated ethenes and ethanes and 3) kinetic parameters of cometabolic processes using a modified Monod model to account for decrease in cell activity over time. Degradation rates for ELW-1 include 5.1 μmol/mg TSS-d for vinyl chloride (VC), 0.2 μmol/mg TSS-d for cis-Dichloroethene (c-DCE), and 4.1 μmol/mg TSS-d for trichloroethene (TCE). Chlorinated ethenes and ethanes were successfully degraded by R. rhodochrous with rates for VC, c-DCE, and TCE at 19.5 μmol/mg TSS-d 3.1 μmol/mg TSS-d and 0.1 μmol/mg TSS-d respectively. Transformation capacity analyses were performed with ELW-1 and TCE giving an estimated capacity of 0.04 mg TCE/mg TSS. A cometabolic transformation capacity model was also successfully fit to experimental data for the transformation of c-DCE by ELW-1. |
| Genre | Thesis |
| Access Condition | http://creativecommons.org/licenses/by/3.0/us/ |
| Topic | chlorinated ethenes |
| Identifier | http://hdl.handle.net/1957/56077 |
