Record Details
Development of Fluorometric Assay for the Determination of β-glucosidase Activity in Brewing Yeasts
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Development of Fluorometric Assay for the Determination of β-glucosidase Activity in Brewing Yeasts |
| Names |
Molitor, Andrew
(creator) Shellhammer, Thomas (advisor) |
| Date Issued | 2015-05-14 (iso8601) |
| Note | Honors Bachelor of Science (HBS) |
| Abstract | Glycosides are natural molecules found in many plants and hold potential to release new flavors and aromas into alcoholic beverages, particularly beer and wine. One way to free these flavors and aromas is by enzymatic hydrolysis catalyzed by βglucosidase. The activities of β-glucosidase are strain and species dependent in yeast as well as other organisms. This study focused on developing a rapid method for the determination of β-glucosidase activity of many brewing yeasts. Knowledge of βglucosidase activity would allow brewers to choose different yeasts depending on their desired effect. Through examining other studies a new method was developed using the substrate 4-MUG and beer-like growth conditions to measure intra-and extracellular yeast β-glucosidase activity. 18 yeasts were measured using this method, confirming previous findings that non-Saccharomyces yeasts tend to exhibit higher enzyme activity than Saccharomyces spp. |
| Genre | Thesis |
| Access Condition | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
| Topic | glycosides |
| Identifier | http://hdl.handle.net/1957/56005 |
