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Development and Characterization of Seven Polymorphic Microsatellite Loci in Bembidion atrocaeruleum (Coleoptera: Carabidae)

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Title Development and Characterization of Seven Polymorphic Microsatellite Loci in Bembidion atrocaeruleum (Coleoptera: Carabidae)
Names O'Callaghan, Matt J. (creator)
Finn, Debra S. (creator)
Williams, Mike (creator)
Sadler, Jonathan P. (creator)
Date Issued 2014-01-01 (iso8601)
Note This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Oxford University Press. The published article can be found at: http://jinsectscience.oxfordjournals.org/.
Abstract We isolated seven polymorphic microsatellite loci from a ground beetle (Bembidion atrocaeruleum, Coleoptera, Carabidae
(Stephens, 1826)) associated with naturally and regularly disturbed floodplain habitat in northwest Europe. Loci were tested on 157 individuals
collected from five distinct habitat patches across two adjacent drainage basins in Wales, United Kingdom, to assess their potential
for revealing population structure across a relatively short spatial extent. Alleles per locus ranged from 4 to 12. For a central representative
population, expected heterozygosity ranged from 0.23 to 0.78 (mean: 0.63), and observed heterozygosity ranged from 0.16
to 0.94 (mean: 0.56). Analysis of molecular variance indicated significant structure among populations, even when one locus potentially
containing null alleles was removed. These loci have the potential to aid the study of dispersal mechanisms of this important riparian
species along and between river corridors, a recurring question in floodplain conservation studies. In addition, given the diversity of
the Bembidion genus, they may have utility in the study of sister species.
Genre Article
Access Condition http://creativecommons.org/licenses/by-nc/3.0/us/
Topic Coleoptera
Identifier O’Callaghan, M. J., Finn, D. S., Williams, M., & Sadler, J. P. (2014). Development and Characterization of Seven Polymorphic Microsatellite Loci in Bembidion atrocaeruleum (Coleoptera: Carabidae). Journal of Insect Science, 14(1), 203. doi:10.1093/jisesa/ieu065

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