Record Details
Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function |
| Names |
He, Yue
(creator) Yam, Candice (creator) Pomraning, Kyle (creator) Chin, Jacqueline S. R. (creator) Yew, Joanne Y. (creator) Freitag, Michael (creator) Oliferenko, Snezhana (creator) |
| Date Issued | 2014-12-15 (iso8601) |
| Note | This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the American Society for Cell Biology. The published article can be found at: http://www.molbiolcell.org/. |
| Abstract | Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in specialized cellular organelles, called lipid droplets. Understanding what determines the cellular amount of neutral lipids and their packaging into lipid droplets is of fundamental and applied interest. Using two species of fission yeast, we show that cycling cells deficient in the function of the ER-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. We demonstrate that these unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Our results suggest that interfering with the CDPDG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway. |
| Genre | Article |
| Access Condition | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ |
| Identifier | He, Y., Yam, C., Pomraning, K., Chin, J. S. R., Yew, J. Y., Freitag, M., & Oliferenko, S. (2014). Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function. Molecular Biology of the Cell, 25(25), 4083-4095. doi:10.1091/mbc.E14-03-0832 |
