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Modeling optimal mineral nutrition for hazelnut micropropagation

ScholarsArchive at Oregon State University

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Title Modeling optimal mineral nutrition for hazelnut micropropagation
Names Hand, Charles (creator)
Maki, Shinya (creator)
Reed, Barbara M. (creator)
Date Issued 2014-11 (iso8601)
Note To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by Springer and can be found at: http://link.springer.com/journal/11240.
Abstract Micropropagation of hazelnut (Corylus avellana
L.) is typically difficult because of the wide variation
in response among cultivars. This study was designed to
determine the required mineral nutrient concentrations for
micropropagation of C. avellana cultivars using a response
surface design analysis. Driver and Kuniyuki Walnut
(DKW) medium mineral nutrients were separated into five
factors: NH₄NO₃, Ca(NO₃)₂, mesos (MgSO₄ and KH₂PO₄),
K₂SO₄, and minor nutrients (boron, copper, manganese,
molybdenum, and zinc) ranging from 0.59 to 29 the
standard DKW medium concentrations with 33 treatments
for use in modeling. Overall quality and shoot length for all
cultivars were influenced by ammonium and nitrate nitrogen,
mesos and minors. Reduced Ca(NO₃)₂ improved
multiplication while higher amounts increased shoot length
for most cultivars. Uptake of nutrients varied among the
cultivars. Calcium and magnesium concentrations were
greater in the shoots that grew well compared to poorly-growing
and control treatments. All five cultivars showed
improved growth on some treatments and the models
indicated that shoots grown on an optimized medium
would be even better. This model indicates that NH₄NO₃,
Ca(NO₃)₂, mesos, and minors all had significant effects on
hazelnut growth and multiplication and should be optimized
in future experiments.
Genre Article
Topic Corylus
Identifier Hand, C., Maki, S., & Reed, B. M. (2014). Modeling optimal mineral nutrition for hazelnut micropropagation. Plant Cell, Tissue and Organ Culture, 119(2), 411-425. doi:10.1007/s11240-014-0544-y

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