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Molecular Survey for the Invasive Leafminer Pest Liriomyza huidobrensis (Diptera: Agromyzidae) in California Uncovers Only the Native Pest Liriomyza langei

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Title Molecular Survey for the Invasive Leafminer Pest Liriomyza huidobrensis (Diptera: Agromyzidae) in California Uncovers Only the Native Pest Liriomyza langei
Names Scheffer, Sonja J. (creator)
Lewis, Matthew L. (creator)
Gaimari, Stephen D. (creator)
Reitz, Stuart R. (creator)
Date Issued 2014-10 (iso8601)
Note To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by the Entomological Society of America and can be found at: http://www.bioone.org/loi/ecen.
Abstract Liriomyza huidobrensis (Blanchard) is a highly destructive invasive leafminer pest
currently causing extensive damage to vegetable and horticultural crops around the world. Liriomyza
langei Frick is a leafminer pest native to California that cannot currently be morphologically distinguished
from L. huidobrensis. We used a DNA-barcoding approach, a published PCR-RFLP method,
and a new multiplex PCR method to analyze 664 flies matching the morphological description of
huidobrensis-langei. We found no evidence for the presence of L. huidobrensis in our extensive samples
from California. In addition to the new molecular method, this work is important because it provides
definitive data that the California “pea leafminer” is currently, and has probably always been, L. langei.
These data will also be important in the event that the highly invasive L. huidobrensis ever becomes
established.
Genre Article
Topic molecular diagnostics
Identifier Scheffer, S. J., Lewis, M. L., Gaimari, S. D., & Reitz, S. R. (2014). Molecular Survey for the Invasive Leafminer Pest Liriomyza huidobrensis (Diptera: Agromyzidae) in California Uncovers Only the Native Pest Liriomyza langei. Journal of Economic Entomology, 107(5), 1959-1964. doi:10.1603/EC13279

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