Record Details
Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency |
| Names |
Reed, Aimee N.
(creator) Izume, Satoko (creator) Dolan, Brian P. (creator) LaPatra, Scott (creator) Kent, Michael (creator) Dong, Jing (creator) Jin, Ling (creator) |
| Date Issued | 2014-06-04 (iso8601) |
| Note | This is the publisher’s final pdf. The published article is copyrighted by the American Society for Microbiology and can be found at: http://jvi.asm.org/. |
| Abstract | Cyprinid herpesvirus 3 (CyHV-3), commonly known as koi herpesvirus (KHV), is a member of the Alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. Our previous study demonstrated that CyHV-3 becomes latent in peripheral white blood cells (WBC). In this study, CyHV-3 latency was further investigated in IgM⁺ WBC. The presence of the CyHV-3 genome in IgM⁺ WBC was about 20-fold greater than in IgM⁻ WBC. To determine whether CyHV-3 expressed genes during latency, transcription from all eight open reading frames (ORFs) in the terminal repeat was investigated in IgM⁺ WBC from koi with latent CyHV-3 infection. Only a spliced ORF6 transcript was found to be abundantly expressed in IgM⁺ WBC from CyHV-3 latently infected koi. The spliced ORF6 transcript was also detected in vitro during productive infection as early as 1 day postinfection. The ORF6 transcript from in vitro infection begins at -127 bp upstream of the ATG codon and ends +188 bp downstream of the stop codon, +20 bp downstream of the polyadenylation signal. The hypothetical protein of ORF6 contains a consensus sequence with homology to a conserved domain of EBNA-3B and ICP4 from Epstein-Barr virus and herpes simplex virus 1, respectively, both members of the Herpesviridae. This is the first report of latent CyHV-3 in B cells and identification of gene transcription during latency for a member of the Alloherpesviridae. |
| Genre | Article |
| Identifier | Reed, A. N., Izume, S., Dolan, B. P., LaPatra, S., Kent, M., Dong, J., & Jin, L. (2014). Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency. Journal of Virology, 88(16), 9297-9309. doi:10.1128/JVI.00990-14 |
