Record Details
Genome-wide mapping of chromatin state of mouse forelimbs
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Genome-wide mapping of chromatin state of mouse forelimbs |
| Names |
Eng, Diana
(creator) Vogel, Walter K. (creator) Flann, Nicholas S. (creator) Gross, Michael K. (creator) Kioussi, Chrissa (creator) |
| Date Issued | 2014-09-10 (iso8601) |
| Note | This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Dove Medical Press Ltd. The published article can be found at: http://www.dovepress.com/open-access-bioinformatics-journal. |
| Abstract | BACKGROUND: Cell types are defined at the molecular level during embryogenesis by a process called pattern formation and created by the selective utilization of combinations of sequence-specific transcription factors. Developmental programs define the sets of genes that are available to each particular cell type, and real-time biochemical signaling interactions define the extent to which these sets are used at any given time and place. Gene expression is regulated through the integrated action of many cis-regulatory elements, including core promoters, enhancers, silencers, and insulators. The chromatin state in developing body parts provides a code to cellular populations that directs their cell fates. Chromatin profiling has been a method of choice for mapping regulatory sequences in cells that go through developmental transitions. RESULTS: We used antibodies against histone H3 lysine 4 trimethylations, a modification associated with promoters and open/active chromatin, histone H3 lysine 27 trimethylations associated with Polycomb-repressed regions, and ribonucleic acid polymerase II associated with transcriptional initiation to identify the chromatin state signature of the mouse forelimb during mid-gestation at embryonic day 12. The families of genes marked included those related to transcriptional regulation and embryogenesis. One-third of the marked genes were transcriptionally active, whereas only a small fraction were bivalent marked. Sequence-specific transcription factors that were activated were involved in cell specification, including bone and muscle formation. CONCLUSION: Our results demonstrate that embryonic limb cells do not exhibit the plasticity of the embryonic stem cells but rather are programmed for a finer tuning for cell lineage specification. |
| Genre | Article |
| Access Condition | http://creativecommons.org/licenses/by-nc/3.0/us/ |
| Topic | mouse genome |
| Identifier | Eng, D., Vogel, W. K., Flann, N. S., Gross, M. K. & Kioussi, C. (2014). Genome-wide mapping of chromatin state of mouse forelimbs. Open Access Bioinformatics, 6, 1-11. doi:10.2147/OAB.S59043 |
