Record Details

Influence of Corexit 9500A and Alaska North Slope Crude oil toxicity to the ammonia-oxidizing bacteria, Nitrosomonas europaea, and the ammonia-oxidizing archaea, Nitrosopumilus maritimus

ScholarsArchive at Oregon State University

Field Value
Title Influence of Corexit 9500A and Alaska North Slope Crude oil toxicity to the ammonia-oxidizing bacteria, Nitrosomonas europaea, and the ammonia-oxidizing archaea, Nitrosopumilus maritimus
Names Schneider, Margaret C. (creator)
Semprini, Lewis (advisor)
Date Issued 2014-06-03 (iso8601)
Note Honors Bachelor of Science (HBS)
Abstract The inhibitory effects of Corexit 9500A, Alaska North Slope Crude oil (ANSC), and mixtures of the two on the ammonia-oxidizing bacteria, Nitrosomonas europaea, and the ammonia-oxidizing archaea, Nitrosopumilus maritimus, were investigated. Corexit 9500A was found to be minimally toxic to both microorganisms with concentrations of 2000 and 3000 ppm yielding a 50% reduction in nitrification activity. The water-associated fraction (WAF) of ANSC was found to be reasonably toxic to N. europaea and N. maritimus with concentrations of 6 and 7 mL per 50 mL media resulting in 50% inhibition. Corexit 9500A:ANSC dispersant enhanced water-associated fraction (DEWAF) mixtures were found to be more toxic to each species as the ratio increased and as larger volumes were added per 50 mL media. ANSC WAF was found to be the most toxic, compared to the DEWAF mixtures, to both N. europaea and N. maritimus when compared on a per COD basis as most of the COD without dispersant present is attributed to low molecular weight aromatic hydrocarbons. These hydrocarbons are, most likely, the cause of the majority of inhibition caused by crude oil. Using dispersants, such as Corexit 9500A, may have a significant effect on microorganism activity and population due to the toxicity of hydrocarbons present in crude oil. In general, similar responses were observed in the different exposures of both microorganisms.
Genre Thesis
Topic Nitrosomonas europaea
Identifier http://hdl.handle.net/1957/52415

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