Record Details
A freshwater cyanophage whose genome indicates close relationships to photosynthetic marine cyanomyophages
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | A freshwater cyanophage whose genome indicates close relationships to photosynthetic marine cyanomyophages |
| Names |
Dreher, Theo W.
(creator) Brown, Nathan (creator) Bozarth, Connie S. (creator) Schwartz, Andrew D. (creator) Riscoe, Erin (creator) Thrash, Cameron (creator) Bennett, Samuel E. (creator) Tzeng, Shin-Cheng (creator) Maier, Claudia S. (creator) |
| Date Issued | 2011-05-23 (iso8601) |
| Note | This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Society for Applied Microbiology and Blackwell Publishing Ltd and published by John Wiley & Sons Ltd. It can be found at: http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291462-2920. |
| Abstract | Bacteriophage S-CRM01 has been isolated from a freshwater strain of Synechococcus and shown to be present in the upper Klamath River valley in northern California and Oregon. The genome of this lytic T4-like phage has a 178,563 bp circular genetic map with 297 predicted protein-coding genes and 33 tRNA genes that represent all 20 amino acid specificities. Analyses based on gene sequence and gene content indicate a close phylogenetic relationship to the “photosynthetic” marine cyanomyophages infecting Synechococcus and Prochlorococcus. Such relatedness suggests that freshwater and marine phages can draw on a common gene pool. The genome can be considered as being comprised of three regions. Region 1 is populated predominantly with structural genes, recognized as such by homology to other T4-like phages and by identification in a proteomic analysis of purified virions. Region 2 contains most of the genes with roles in replication, recombination, nucleotide metabolism and regulation of gene expression, as well as 5 of the 6 signature genes of the photosynthetic cyanomyophages (hli03, hsp20, mazG, phoH and psbA; cobS is present in Region 3). Much of Regions 1 and 2 are syntenous with marine cyanomyophage genomes, except that a segment encompassing Region 2 is inverted. Region 3 contains a high proportion (85%) of genes that are unique to S-CRM01, as well as most of the tRNA genes. Regions 1 and 2 contain many predicted late promoters, with a combination of CTAAATA and ATAAATA core sequences. Two predicted genes that are unusual in phage genomes are homologs of cellular spoT and nusG. |
| Genre | Article |
| Topic | Myoviridae |
| Identifier | Dreher, T. W., Brown, N., Bozarth, C. S., Schwartz, A. D., Riscoe, E., Thrash, C., Bennett, S. E., Tzeng, S.-C. and Maier, C. S. (2011), A freshwater cyanophage whose genome indicates close relationships to photosynthetic marine cyanomyophages. Environmental Microbiology, 13: 1858–1874. doi:10.1111/j.1462-2920.2011.02502.x |
