Record Details
Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterization of Protein Adducts Using an Aldehyde/Keto Reactive Probe in Combination with Mass Spectrometry
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterization of Protein Adducts Using an Aldehyde/Keto Reactive Probe in Combination with Mass Spectrometry |
| Names |
Maier, Claudia S.
(creator) Chavez, Juan (creator) Wang, Jing (creator) Wu, Jianyong (creator) |
| Date Issued | 2010 (iso8601) |
| Note | This is an author's manuscript, as accepted by the publisher. The published chapter 16 is copyrighted by Elsevier. |
| Abstract | This chapter describes a mass spectrometry-based strategy that facilitates the unambiguous identification and characterization of proteins modified by lipid peroxidation-derived 2-alkenals. The approach employs a biotinylated hydroxyl amine derivative as an aldehyde/keto reactive probe in conjunction with selective enrichment and tandem mass spectrometric analysis. Methodological details are given for model studies involving a distinct protein and 4-hydroxy-2-nonenal (HNE). The method was also evaluated for an exposure study of a cell culture system with HNE that yielded the major protein targets of HNE in human monocytic THP-1 cells. The application of the approach to complex biological systems is demonstrated for the identification and characterization of endogenous protein targets of aldehydic lipid peroxidation products present in cardiac mitochondria. |
| Genre | Book chapter |
| Identifier | Maier, C. S., Chavez, J., Wang, J., & Wu, J. (2010). Protein adducts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry. Methods in Enzymology, 473, 305-330. doi:10.1016/S0076-6879(10)73016-0 |
