Record Details
Permeability of Various Thyroid Hormone Derivatives and Discovery of a Novel Metabolite
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Permeability of Various Thyroid Hormone Derivatives and Discovery of a Novel Metabolite |
| Names |
Kirkemo, Lisa
(creator) Ahern, Kevin (advisor) |
| Date Issued | 2014-05-22 (iso8601) |
| Note | Honors Bachelor of Science (HBS) |
| Abstract | In this investigation, six different endogenous thyroid hormone derivatives—T₄, T₃, TA₄, TA₃, T₁-Amine, and N-Acetylated T₁-Amine—were tested to gain insight into their permeability coefficients and efflux ratios across a monolayer of Madin-Darby Canine Kidney (MDCK II) cells. The results indicate that T₃ and T₄ were unable to traverse the membrane due to lack of compatible membrane bound transporters, which supports that the transport of both T₃ and T₄ into their cellular targets relies on membrane proteins. The data also suggested that T₁-Amine and N-Acetylated T₁-Amine may cross the plasma membrane by an active transport mechanism, while the remaining derivatives express efflux ratios indicative of a passive diffusion mechanism. Incubating N-Acetylated T₁-Amine with MDCK II cells produced a novel metabolite, which was identified as a sulfated form of N-Acetylated T₁-Amine. |
| Genre | Thesis |
| Topic | Thyroid hormone derivatives |
| Identifier | http://hdl.handle.net/1957/50854 |
