Record Details
Pseudomonas protegens Pf-5 Causes Discoloration and Pitting of Mushroom Caps Due to the Production of Antifungal Metabolites
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Pseudomonas protegens Pf-5 Causes Discoloration and Pitting of Mushroom Caps Due to the Production of Antifungal Metabolites |
| Names |
Henkels, Marcella D.
(creator) Kidarsa, Teresa A. (creator) Shaffer, Brenda T. (creator) Goebel, Neal C. (creator) Rangel, Lorena I. (creator) Davis, Edward W., II (creator) Zabriskie, T. Mark (creator) Loper, Joyce E. (creator) et al. (creator) |
| Date Issued | 2014-07 (iso8601) |
| Note | To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The article was published by the American Phytopathological Society and is in the public domain. The published article can be found at: http://apsjournals.apsnet.org/loi/mpmi. |
| Abstract | Bacteria in the diverse Pseudomonas fluorescens group include rhizosphere inhabitants known for their antifungal metabolite production and biological control of plant disease, such as Pseudomonas protegens Pf-5, and mushroom pathogens, such as Pseudomonas tolaasii. Here, we report that strain Pf-5 causes brown, sunken lesions on peeled caps of the button mushroom (Agaricus bisporus) that resemble brown blotch symptoms caused by P. tolaasii. Strain Pf-5 produces six known antifungal metabolites under the control of the GacS/GacA signal transduction system. A gacA mutant produces none of these metabolites and did not cause lesions on mushroom caps. Mutants deficient in the biosynthesis of the antifungal metabolites 2,4-diacetylphloroglucinol and pyoluteorin caused less-severe symptoms than wild-type Pf-5 on peeled mushroom caps, whereas mutants deficient in the production of lipopeptide orfamide A caused similar symptoms to wild-type Pf-5. Purified pyoluteorin and 2,4-diacetylphloroglucinol mimicked the symptoms caused by Pf-5. Both compounds were isolated from mushroom tissue inoculated with Pf-5, providing direct evidence for their in situ production by the bacterium. Although the lipopeptide tolaasin is responsible for brown blotch of mushroom caused by P. tolaasii, P. protegens Pf-5 caused brown blotch-like symptoms on peeled mushroom caps through a lipopeptide-independent mechanism involving the production of 2,4-diacetylphloroglucinol and pyoluteorin. |
| Genre | Article |
| Identifier | Henkels, M. D., Kidarsa, T. A., Shaffer, B. T., Goebel, N. C., Burlinson, P., Mavrodi, D. V., ... & Loper, J. E. (2014). Pseudomonas protegens Pf-5 Causes Discoloration and Pitting of Mushroom Caps Due to the Production of Antifungal Metabolites. Molecular Plant-Microbe Interactions, 27(7), 733-746. doi:10.1094/MPMI-10-13-0311-R |
