Record Details
Simultaneous HPLC-DAD quantification of vitamins A and E content in raw, pasteurized, and UHT cow’s milk and their changes during storage
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Simultaneous HPLC-DAD quantification of vitamins A and E content in raw, pasteurized, and UHT cow’s milk and their changes during storage |
| Names |
Chotyakul, Nattaporn
(creator) Pateiro-Moure, Miriam (creator) Saraiva, Jorge Alexandre (creator) Torres, J. Antonio (creator) Pérez-Lamela, Concepción (creator) |
| Date Issued | 2014-04 (iso8601) |
| Note | This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer and can be found at: http://link.springer.com/journal/217. |
| Abstract | An improved extraction and HPLC method for the simultaneous extraction and quantitation of retinol, α-tocopherol, α-tocotrienol and β-carotene was developed to analyze commercial whole/semi-skim/skim samples of raw/pasteurized/UHT milk in transparent plastic/glass bottles and Tetra Brik™ containers. The sample preparation method required prior saponification at 40ºC for 15 min followed by n-hexane extraction. An isocratic acetonitrile:methanol (65:35 v/v) mobile phase and UV detection were chosen for HPLC quantification. The liposoluble vitamin content in raw, pasteurized conventional/organic, and UHT milk ranged 0.055-5.540 (retinol), 0.135-1.410 (α-tocopherol), and 0.040-0.850 mg/L (β-carotene). No significant differences (p>0.05) were observed on losses of retinol, α-tocopherol, and β-carotene content in UHT whole milk after 5 days at 4ºC in the dark. After 14 days at 4ºC in the dark the contents of retinol, α-tocopherol, and β-carotene remained higher in milk with higher fat content and were higher in unopened containers. In UHT whole milk samples containing 0.02% NaN₃, retinol (33%) and α-tocopherol (11%) but not β-carotene (2%) decreased significantly (p<0.05). |
| Genre | Article |
| Topic | Cow’s milk |
| Identifier | Chotyakul, N., Pateiro-Moure, M., Saraiva, J. A., Torres, J. A., & Pérez-Lamela, C. (2014). Simultaneous HPLC–DAD quantification of vitamins A and E content in raw, pasteurized, and UHT cow’s milk and their changes during storage. European Food Research and Technology, 238(4), 535-547. doi:10.1007/s00217-013-2130-7 |
