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The fate of specific organisms in a receiving stream

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Title The fate of specific organisms in a receiving stream
Names Henshaw, Thomas Lamond (creator)
Phillips, Don C. (advisor)
Date Issued 1963-05-01 (iso8601)
Note Graduation date: 1963
Abstract One of the major items in any program of municipal sanitation
is the double problem of water supply and waste water disposal. Since
streams are the general source of water supply and also the final receptacle
for sewage, this dual use of the stream often leads to complications.
Sewage and water treatment are considered man-made lines
of defense against the passage of bacterial pollution. Bacterial self-purification can be considered a natural barrier. This thesis presents
a study of the die-off of certain specific organisms in a stream receiving
treated sewage.
The study was accomplished by drawing samples of the receiving
stream at specified sampling stations and determining the density
of the organisms Escherichia-coli and fecal streptococci, and the total
number of viable cells present in the sample. All bacterial counts
were made using the membrane filter technique except for the total counts, which were obtained using the standard pour -plate method.
Curves showing bacterial pollution versus river miles were obtained
relating the die-off of the bacteria to miles below the sewer outfall.
Conclusions reached from this study were: (1) Both the
Escherichia-coli or fecal streptococci counts yielded a good index
of stream pollution, however, the E-coli colonies proved to be the
easier of the two to count. (2) The membrane filter technique offered
reliability and ease in counting for large numbers of samples. (3) Increased
temperature caused increased bacterial die off in incubated
samples of river water. (4) For both 10 °C and 20 °C temperatures,
the E-coli were noted to die off more rapidly than the fecal streptococci.
(5) In Mary's River, during the winter months, 64 percent of
the E-coli died-off in a distance of five and a half miles. Within the
same distance, 71 percent of the fecal streptococci died-off.
Genre Thesis/Dissertation
Topic Water -- Pollution
Identifier http://hdl.handle.net/1957/48802

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