Record Details
Field | Value |
---|---|
Title | Alternative splicing in the obligate biotrophic oomycete pathogen, Pseudoperonospora cubensis |
Names |
Burkhardt, Alyssa
(creator) Buchanan, Alex (creator) Cumbie, Jason S. (creator) Savory, Elizabeth, A. (creator) Chang, Jeff H. (creator) Day, Brad (creator) |
Date Issued | 2014-06-05 (iso8601) |
Note | Suggested citation: "Burkhardt, Alyssa; Buchanan, Alex; Cumbie, Jason S.; Savory, Elizabeth, A.; Chang, Jeff H.; Day, Brad (2014): Alternative splicing in the obligate biotrophic oomycete pathogen, Pseudoperonospora cubensis. Oregon State University Libraries. Dataset. http://dx.doi.org/10.7267/N9TD9V7M" |
Abstract | We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer paired-end (PE) sequencing on four channels of an Illumina HiSeq. As a first step in the characterization of alternative splicing, we used the RNA-Seq reads to reannotate the Ps. cubensis genome, which is deposited as a gff file, along with a relational table that associates the most current annotation to the previous Ps. cubensis annotation. |
Genre | Dataset |
Access Condition | http://creativecommons.org/publicdomain/zero/1.0/ |
Topic | RNA-Seq |
Identifier | SRA: SRP042019 |