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A Rapid Method to Assess Grape Rust Mites on Leaves and Observations from Case Studies in Western Oregon Vineyards

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Title A Rapid Method to Assess Grape Rust Mites on Leaves and Observations from Case Studies in Western Oregon Vineyards
Names Schreiner, R. Paul (creator)
Skinkis, Patricia A. (creator)
Dreves, Amy J. (creator)
Date Issued 2014-02 (iso8601)
Note To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by the American Society for Horticultural Science and can be found at: http://horttech.ashspublications.org/.
Abstract The grape rust mite [Calepitrimerus vitis (Acari: Eriophyidae)] is an
important pest of grapevines (Vitis sp.) in grape-growing regions around the world.
A rapid method for extracting eriophyoid mites was adapted from earlier studies to
provide integrated pest management (IPM) consultants and commercial growers
with a practical, efficient, and reliable tool to monitor grape rust mites in vineyards
and nursery stock vines. The rinse in bag (RIB) method allows quick extraction of
mites from young shoots or from leaves using 35% to 70% ethanol or isopropanol in
a sealable plastic bag. The RIB method recovered 85% of grape rust mites from
single leaves in the first rinse. The method is useful to estimate grape rust mites on
young shoots (≤10 cm length), although recovery of grape rust mites (average
ranging from 35% to 81%) was lower because of a higher density of trichomes on
young shoots as compared with leaf samples. The RIB method was not effective to
assess grape rust mites within dormant buds, so a separate method using a blender to
disrupt tissues and extract mites in alcohol was developed. The RIB method was
used to determine grape rust mite abundance with leaf symptoms in commercial
vineyards and nursery stock vines. The earliest visible symptom of grape rust mite
damage on leaves in the summer was the development of stippling that is distinct
from the type of damage caused by other grapevine pests. The stippling is described
as numerous clear zones of small diameter (resembling pinholes) that are visible
when a leaf is backlit. The severity of stippling was related to the number of grape
rust mites present on leaves, with >600 occurring on leaves with severe stippling
symptoms. In commercial vineyard case studies, the RIB method was used over two
seasons and revealed that grape rust mite populations remained on leaves until
postharvest, and foliar applications of wettable sulfur reduced grape rust mite
populations on leaves.
Genre Article
Topic Calepitrimerus vitis
Identifier Schreiner, R. P., Skinkis, P. A., & Dreves, A. J. (2014). A Rapid Method to Assess Grape Rust Mites on Leaves and Observations from Case Studies in Western Oregon Vineyards. HortTechnology, 24(1), 38-47.

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