Record Details

Morphology of epididymal spermatozoa from inbred and linecross bulls

ScholarsArchive at Oregon State University

Field Value
Title Morphology of epididymal spermatozoa from inbred and linecross bulls
Names Turner, H. A. (creator)
Bogart, Ralph (advisor)
Date Issued 1965-06-09 (iso8601)
Note Graduation date: 1966
Abstract Data were presented on epididymal spermatozoa from 29 inbred
and linecross bulls raised at Oregon State University in connection
with the Western Regional Beef Cattle Breeding Project, W-1. The
inbred bulls came from four inbred lines. Three of these inbred
lines were Herfords and the other line was Angus. The 21 linecross
bulls were produced from reciprocal crosses of the three Hereford
lines.
The bulls were put on test at 450 pounds. They were fed one
part concentrate to two parts roughage up to 800 pounds at which time
the concentrates were increased. They were slaughtered at 1000
pounds.
The epididymides were stripped from the testes at the time of
slaughter and sperm were obtained from the head and tail of the epididymides
for making slides. An eosin-fast green differential stain
was used to make slides for characterizing the spermatozoa as dead
or alive and for detecting physiological abnormalities. Two slides
were made from each location on the epididymis and labeled as head
or tail and left or right. This made 16 slides per bull.
The slides were then studied and 200 spermatozoa were counted
on each slide. Only ten to 25 spermatozoa were counted in one spot
in order to obtain an overall picture of each slide. The number of
live, dead, normal, and types of abnormals were recorded. The different
types of abnormals counted and recorded were neck beads,
tail beads, tailless heads, coiled tails, and bent tails.
An analysis of variance was run for breeding types, sides, and
locations and all possible interactions between these variables. Statistical
significance was determined by use of the F test.
Means were calculated for breeding types and locations. To
estimate general combining ability effects, means of lines I, II, or
III combined with each of the other two lines are compared. To estimate
specific combining ability, means of I x II, I x III, and II x III
are compared.
The analysis of variance indicated that there were statistically
significant differences between breeding types for live spermatozoa,
tail beads, and bent tails. Since a particular breeding type that was
superior in one characteristic was also inferior in another, there was
no generally superior breeding type.
Genre Thesis/Dissertation
Topic Spermatozoa
Identifier http://hdl.handle.net/1957/47937

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