Record Details
The effects of univalent cations on the inductive formation of nitrate reductase
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | The effects of univalent cations on the inductive formation of nitrate reductase |
| Names |
Nitsos, Ronald Eugene
(creator) Evans, Harold J. (advisor) |
| Date Issued | 1966-05-12 (iso8601) |
| Note | Graduation date: 1966 |
| Abstract | An investigation was undertaken to determine whether potassium is indeed necessary for the in vivo synthesis of a specific protein. The effects of univalent cations on the inductive formation of nitrate reductase and on the level of activity of representative non-adaptive enzyme are presented. Cultures of Neurospora crassa were grown in media without added univalent cations and the mycelia then transferred to induction media containing different univalent cations. The inductive formation of nitrate reductase was followed over a four-hour period. During the same period of incubation assays for the activity of the constitutive enzyme pyruvic kinase were conducted. Results of nitrate reductase assays during the induction period indicate that the formation of this enzyme is dependent upon an adequate concentration of potassium in the medium. Sodium, lithium, and ammonium could not substitute for potassium in the inductive formation of this enzyme. Rubidium was the only univalent cation besides potassium that partially functioned in the synthesis of nitrate reductase. Studies of pyruvic kinase activity during the in duction period indicate that its activity remained nearly constant. It appears that conditions were optimum for the rapid synthesis of nitrate reductase, but the rate of protein synthesis in general was not greatly changed during the induction period of nitrate reductase. |
| Genre | Thesis/Dissertation |
| Topic | Plants, Effect of potassium on |
| Identifier | http://hdl.handle.net/1957/47826 |
