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Studies on the methodology of crossing and selfing in Lolium multiflorum

ScholarsArchive at Oregon State University

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Title Studies on the methodology of crossing and selfing in Lolium multiflorum
Names Bergman, Jerald Wilmer (creator)
Frakes, Rod V. (advisor)
Date Issued 1967-12-08 (iso8601)
Note Graduation date: 1968
Abstract Studies were conducted to determine the effectiveness of a
biological pollen barrier of rye, Secale cereale, in preventing out-crossing between Lolium multiflorum genotypes in each of the 300
crossing blocks established in this experiment.
Fluorescent tests of seeds of open-pollinated and single-crossed progeny of four annual ryegrass sources were used to
determine the effectiveness of the rye barrier in preventing crossing
between crossing blocks. The average percentage of fluorescence
of open-pollinated progeny in 30 crossing blocks containing
supposedly only non-fluorescing plants was 16.3 percent. The
open-pollinated and single-crossed progeny in 46 other crossing
blocks were highly correlated and did not differ significantly from
each other in percent fluorescence. The percent fluorescence of
each crossing block was mainly due to intra-crossing block pollen
rather than inter-crossing block or foreign pollen. The results
indicate that an eight foot barrier of rye is effective in preventing
crossing between the annual ryegrass crossing blocks.
Self-fertility of Lolium multiflorum was generally low, but
self fertility estimates ranged from 0 to 87 percent. The results
revealed that self-fertility data obtained by germination rather than
seed count may give more accurate self-fertility estimates, especially
when individual plants and their differences are being investigated.
The average seed set under white parchment bags was not
significantly lower than the average seed set obtained with bag-type
dialysis tubing. The use of dialysis tubing as a substitute for white
parchment bags in bagging plants would be of little, if any, benefit
to improve seed set under bag in Lolium multiflorum.
Genre Thesis/Dissertation
Topic Ryegrasses
Identifier http://hdl.handle.net/1957/47363

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