Record Details
Mice Fed Rapamycin Have an Increase in Lifespan Associated with Major Changes in the Liver Transcriptome
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Mice Fed Rapamycin Have an Increase in Lifespan Associated with Major Changes in the Liver Transcriptome |
| Names |
Fok, Wilson C.
(creator) Chen, Yidong (creator) Bokov, Alex (creator) Zhang, Yiqiang (creator) Salmon, Adam B. (creator) Diaz, Vivian (creator) Javors, Martin (creator) Wood, William H., III (creator) Zhang, Yongqing (creator) Becker, Kevin G. (creator) Pérez, Viviana I. (creator) Richardson, Arlan (creator) |
| Date Issued | 2014-01-07 (iso8601) |
| Note | This is the publisher’s final pdf. The article was published by the Public Library of Science and is in the public domain. The published article can be found at: http://www.plosone.org/. |
| Abstract | Rapamycin was found to increase (11% to 16%) the lifespan of male and female C57BL/6J mice most likely by reducing the increase in the hazard for mortality (i.e., the rate of aging) term in the Gompertz mortality analysis. To identify the pathways that could be responsible for rapamycin’s longevity effect, we analyzed the transcriptome of liver from 25-month-old male and female mice fed rapamycin starting at 4 months of age. Few changes (<300 transcripts) were observed in transcriptome of rapamycin-fed males; however, a large number of transcripts (>4,500) changed significantly in females. Using multidimensional scaling and heatmap analyses, the male mice fed rapamycin were found to segregate into two groups: one group that is almost identical to control males (Rapa-1) and a second group (Rapa-2) that shows a change in gene expression (>4,000 transcripts) with more than 60% of the genes shared with female mice fed Rapa. Using ingenuity pathway analysis, 13 pathways were significantly altered in both Rapa-2 males and rapamycin-fed females with mitochondrial function as the most significantly changed pathway. Our findings show that rapamycin has a major effect on the transcriptome and point to several pathways that would likely impact the longevity. |
| Genre | Article |
| Access Condition | http://creativecommons.org/publicdomain/zero/1.0/ |
| Identifier | Fok WC, Chen Y, Bokov A, Zhang Y, Salmon AB, et al. (2014) Mice Fed Rapamycin Have an Increase in Lifespan Associated with Major Changes in the Liver Transcriptome. PLoS ONE 9(1): e83988. doi:10.1371/journal.pone.0083988 |
