Record Details
Field | Value |
---|---|
Title | Some factors affecting aphid transmission of cucumber mosaic virus |
Names |
Stimmann, Michael W.
(creator) Swenson, Knud George (advisor) |
Date Issued | 1967-04-21 (iso8601) |
Note | Graduation date: 1967 |
Abstract | The frequency of aphid transmission of cucumber mosaic virus from diseased plants at different times after inoculation was determined. A cycle of infectious virus was found. Primary leaves of lima bean plants were inoculated mechanically when the first trifoliate leaf appeared and were kept at different temperatures. At 24° C day- and 18° C night-temperature, highest transmission frequency from the first trifoliate leaf occurred after 9 to 12 days. The peak of transmission from the second trifoliate leaf was less than half that from the first trifoliate leaf. At 32° C day- and 26° C night-temperature, the peak of transmission frequency from the first trifoliate leaf occurred after 4 to 5 days. A higher peak was reached at the lower temperature. No differences in susceptibility to the virus by aphid transmission were found when the plants were kept at 15° C, 24° C, and 30° C for two days immediately before inoculation. Plants kept in the dark for two days immediately before and after inoculation did not differ in susceptibility from plants that received 16 hours of light per day. The number of infected plants usually increased with higher postinoculation temperatures. The Ouchterlony agar double diffusion test was used to identify the virus. Partially purified preparations from infected plants gave a positive reaction when tested with cucumber mosaic virus antiserum. There was no reaction to similarly treated, healthy-plant preparations. |
Genre | Thesis/Dissertation |
Topic | Cucumber mosaic virus |
Identifier | http://hdl.handle.net/1957/47043 |