Record Details

Some factors affecting aphid transmission of cucumber mosaic virus

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Title Some factors affecting aphid transmission of cucumber mosaic virus
Names Stimmann, Michael W. (creator)
Swenson, Knud George (advisor)
Date Issued 1967-04-21 (iso8601)
Note Graduation date: 1967
Abstract The frequency of aphid transmission of cucumber mosaic virus
from diseased plants at different times after inoculation was determined.
A cycle of infectious virus was found. Primary leaves
of lima bean plants were inoculated mechanically when the first trifoliate
leaf appeared and were kept at different temperatures. At
24° C day- and 18° C night-temperature, highest transmission
frequency from the first trifoliate leaf occurred after 9 to 12 days.
The peak of transmission from the second trifoliate leaf was less
than half that from the first trifoliate leaf. At 32° C day- and 26° C
night-temperature, the peak of transmission frequency from the
first trifoliate leaf occurred after 4 to 5 days. A higher peak was
reached at the lower temperature.
No differences in susceptibility to the virus by aphid transmission
were found when the plants were kept at 15° C, 24° C, and
30° C for two days immediately before inoculation. Plants kept in
the dark for two days immediately before and after inoculation did
not differ in susceptibility from plants that received 16 hours of light
per day. The number of infected plants usually increased with higher
postinoculation temperatures.
The Ouchterlony agar double diffusion test was used to identify
the virus. Partially purified preparations from infected plants gave
a positive reaction when tested with cucumber mosaic virus antiserum.
There was no reaction to similarly treated, healthy-plant
preparations.
Genre Thesis/Dissertation
Topic Cucumber mosaic virus
Identifier http://hdl.handle.net/1957/47043

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