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Neutral lipid metabolism in the fat body of the larva of the fleshfly, Sarcophaga bullata

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Title Neutral lipid metabolism in the fat body of the larva of the fleshfly, Sarcophaga bullata
Names Sun, Grace Y. (creator)
Brookes, V. J. (advisor)
Date Issued 1966-08-04 (iso8601)
Note Graduation date: 1967
Abstract The
purpose
of
this
study
was
to
characterize
the
neutral
lipids
of
the
fat
body
of
the
larva
of
the
fleshfly
Sarcophaga
bullata
and
to
examine
the
means
by
which
these
lipids
are
synthesized.
The
weight
of
the
larva,
the
fat
body
as
well
as
its
lipid
content
were
examined
in
relationship
to
the
growth
of
the
larva.
The
average
larva
attained
maximum
body
weight
of
about
220
mg
in
70
hours.
However,
fat
body
lipid
continued
to
increase
in
weight
after
the
feeding
stage
of
the
larva
during
which
time
there
was
a
small
decrease
in
total
larval
wet
weight.
The
fat
body
lipid
comprised
mainly
of
the
neutral
lipid
and
triglyceride
formed
the
major
portion
of
the
neutral
lipid.
Approximately
60%
of
the
total
fatty
acids
analysed
was
found
to
be
unsaturated
and
an
increase
in
unsaturated
fatty
acid
content
was
found
in
the
fat
body
of
the
nine
day
old
larvae.
The
major
portion
of
fatty
acids
in
the
fat
body
as
analysed
by
gas-liquid
chromatography
were palmitic,
palmitoleic,
stearic,
oleic
and
linoleic
acids.
Very
small
amount
of
acetate-1-C¹⁴ was
incorporated
into
the
fat
body
lipid.
Palmitate-1-C¹⁴, on
the
other
hand,
was
more
extensively
incorporated.
Most
of
the
radioactivity
which
was
incorporated
into
the
neutral
lipid
was
present
in
the
three
glyceride
fractions,
of
which
the
diglycerides
showed
the
highest
specific
activity.
It
is
suggested
that
diglyceride
could
play
an
important
role
in
lipid
transport
in
the
insect
system (13, 14). Incorporation
of
palmitate-1-C¹⁴ into
fat
body
lipid
during
different
time
intervals
of
incubation
indicated
that
there
was
a
very
rapid
uptake
of
the
radioactive
material
in
the
first
30
minutes.
Comparison
of
palmitate-1-C¹⁴ incorporation
between
the
three
day
and
seven
day
old
larvae
showed
that
larvae
at
their
prepupal
stage
were
still
able
to
incorporate
long
chain
fatty
acids
but
to a
somewhat
less
extent.
Fatty
acids
of
neutral
lipid
were
separated
into
saturated
and
unsaturated
components
on
thin-layer plates.
After
incubation
of
fat
body
of
three
day
old
larvae
with
palmitate-1-C¹⁴, the
ratio
of
the
distribution
of
the
label
between
unsaturated
and
saturated
fatty
acid
was
approximately 60-40,
This
ratio
was
achieved
within
ten
minutes
of
incubation.
In
seven day
old
larvae,
most
of
the
label
was
found
in
the
saturated
fraction.
Genre Thesis/Dissertation
Topic Lipids -- Metabolism
Identifier http://hdl.handle.net/1957/47011

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