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Effects of temperature on the development of Fasciola hepatica larval forms in Lymnea columella

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Title Effects of temperature on the development of Fasciola hepatica larval forms in Lymnea columella
Names Wall, David Jerome (creator)
Pratt, Ivan (advisor)
Date Issued 1967-06-26 (iso8601)
Note Graduation date: 1968
Abstract Lymnea columella snails were exposed to 3-5 newly emerged
Fasciola hepatica miracidia. The procedure insured 75-100 percent
infection of the host. Groups of 40 each exposed snails were placed
in controlled temperature containers and maintained at the following
series of temperatures: 8°, 15°, 20°, 25°, 30°, and 35° C. Two
exposed snails were removed from each container one day after exposure
and every seventh day thereafter. The removed snails were
fixed in 10 percent neutral formalin and prepared for serial sectioning
and staining. The stained sections were checked for the presence
of larval types, i.e. sporocysts, rediae, and cercariae.
No larval forms were seen in snails maintained at 8°, 15° nor
at 35° C. Snails died in the latter after six days. The rediae were
seen in snails maintained for 14 days at 25° and at 30° C. Redial development
was seen at 20° C, 29 days after exposure and at 14 days
in snails cultured at 25° and 30° C, At 30° C the larvae maintained
accelerated growth for 21-25 days after exposure before the death
of the snail hosts.
The first motile cercariae were seen 45-47 days after exposure
at 25° C and by 60-65 days at 20° C.
Genre Thesis/Dissertation
Topic Liver flukes
Identifier http://hdl.handle.net/1957/46770

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