Record Details
Terminal methylation reactions in Saccharomyces cerevisiae
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Terminal methylation reactions in Saccharomyces cerevisiae |
| Names |
Botsford, James Lawrence
(creator) Parks, Leo W. (advisor) |
| Date Issued | 1968-04-26 (iso8601) |
| Note | Graduation date: 1968 |
| Abstract | A cell-free system for assessing the role of S-adenosyl-methionine in the methylation of homocysteine deriving the methyl group from serine has been developed. No role for S-adenosyl-methionine could be shown in the in vitro reaction The enzyme serine transhydroxymethylase has been studied in crude and partially purified cell extracts. Evidence was obtained that this activity provides the one carbon fragment for the methylation of homocysteine. The activity was found to be inhibited by S-adenosyl-methionine and methionine. When cells are cultured in excess methionine, the methylation of homocysteine in cell-free extracts is not affected. Serine trans-hydroxymethylase is repressed to a limited degree. The enzyme S-adenosylmethionine:homocysteine methyltransferase is induced under these conditions. |
| Genre | Thesis/Dissertation |
| Topic | Methylation |
| Identifier | http://hdl.handle.net/1957/46584 |
