Record Details
Dissecting DNA Damage Responses in Arabidopsis: A High-Throughput Sequencing Approach
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Dissecting DNA Damage Responses in Arabidopsis: A High-Throughput Sequencing Approach |
| Names |
Brar, Anantnoor
(creator) Hays, John B. (advisor) |
| Date Issued | 2014-03-18 (iso8601) |
| Note | Honors Bachelor of Science (HBS) |
| Abstract | A unique, permanent root-growth arrest phenotype was observed in UV-B irradiated Arabidopsis thaliana roots lacking the DNA damage response kinase ATR. Segregation analysis suggests the dependence of this phenotype on another, unidentified gene, termed ursu. High-throughput sequencing technologies were used to map and identify the causal ursu mutation. Illumina HiSeq 2000 short sequence reads were analyzed using bioinformatics command line tools for Next-Generation Mapping (NGM) and Mapping and Alignment with Short Sequences (MASS) programs. Several putative candidate genes were identified, including auxin response factor 13 (arf13) and resistance to Peronospora parasitica protein 8 (rpp8). Sanger sequencing verified the presence of a single nucleotide polymorphism (SNP) and a frameshift mutation caused by a 26-bp deletion in arf13 in ursu atr plants. Although additional sequencing and complementation tests are still necessary, a causative mutation in arf13 could provide clues linking the DNA damage response kinase ATR with the key growth and development phytohormone auxin. |
| Genre | Thesis |
| Topic | mapping-by-sequencing |
| Identifier | http://hdl.handle.net/1957/46723 |
