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Fertility in the domestic hen as related to the events of the reproductive cycle

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Title Fertility in the domestic hen as related to the events of the reproductive cycle
Names Hughes, Buddy Lee, 1942- (creator)
Parker, Jesse E. (advisor)
Date Issued 1970-01-30 (iso8601)
Note Graduation date: 1970
Abstract To observe the effects of oviposition as related to time of insemination
on fertility, four trials were conducted in which individually
caged White Leghorn hens were artificially inseminated with 0.05 cc
of undiluted mixed semen obtained from 4 to 5 cockerels. These inseminations
were made at 9:45 a.m. , 10:00 a.m. and 12:00 noon.
Beginning three hours prior to insemination and continuing to 29 hours
after insemination, the time of oviposition of each hen was recorded.
Eggs were saved and incubated for two periods, 2 to 9 days and 10 to
17 days following insemination. When the hens were inseminated
within three hours before oviposition or two hours after oviposition
fertility decreased; and the magnitude of this decrease was inversely
related to the time interval between insemination and oviposition.
Hens laying 25 to 27 hours after insemination also showed a tendency
toward low fertility; and the fact that a high percentage of these hens
also laid within two hours prior to insemination may have contributed
to this low fertility. A hard-shelled egg in the oviduct at the time of
insemination did not affect the resulting fertility unless that egg was
laid within three hours after insemination. Ovulation or an egg in
the anterior oviduct as far back as the magnum at the time of insemination
apparently had little or no influence on the resulting fertility.
There was a high correlation between duration of fertility and percentage
of fertility.
To observe the effects of oxytocin on fertility, hens with hard-shelled
eggs in the uteri were either given an intravenous injection of
5 I.U. of oxytocin and inseminated after they laid, or inseminated
and then forced to lay by an intravenous injection of 5 I.U. of oxytocin.
Hens without a hard-shelled egg in the uterus were either given an
intravenous injection of oxytocin and then inseminated, or inseminated
with semen diluted with 0.1 cc (2 I.U.) of oxytocin per cc of semen.
Hens which had laid several hours previously and did not receive
oxytocin served as controls. Hens injected with oxytocin or inseminated
with semen containing oxytocin had lower fertility than the
controls.
Addition of 0.5 I.U. or 1.0 I.U. of oxytocin per cc of saline
solution used as a diluent for observing spermatozoa motility greatly
reduced spermatozoa motility. Addition of 2 I.U. or 4 I.U. of oxytocin
per cc of semen stored at 5°C caused temporary immobilization of the spermatozoa; and when 12 I.U. of oxytocin were added the
spermatozoa did not regain their motility.
Genre Thesis/Dissertation
Topic Poultry -- Breeding
Identifier http://hdl.handle.net/1957/46239

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