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Culture and nutritional requirements of sod webworms on a meridic diet (Lepidoptera : Pyralidae : Crambinae)

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Title Culture and nutritional requirements of sod webworms on a meridic diet (Lepidoptera : Pyralidae : Crambinae)
Names Dupnik, Theodore David (creator)
Kamm, J. A. (advisor)
Date Issued 1969-08-15 (iso8601)
Note Graduation date: 1970
Abstract Attempts were made to rear Crambus topiarius Zeller,
Euchromius ocellus (Haworth), Crambus bonifatellus (Hulst) and
Crambus trisectus (Walker) on non-aseptic meridic diets containing
wheat germ or pinto beans as basic constituents. Various methods
of diet presentation and types of rearing containers were used. Shell
vials and plastic cups used as rearing containers were unsuitable for
G. topiarius. The reduction of toxic vapors emitted by anti-microbial
compounds permitted six successive generations of E. ocellus to be
reared on a wheat germ diet modified from one reported in the literature.
The successful cultural methods employed 1 cc cubes of a
meridic diet and the use of 3/4 oz paper cups as rearing containers.
Attempts to rear C. bonifatellus on a meridic diet were not
successful.
C. trisectus was found to require beta-sitosterol in the diet for
larval growth. Sucrose in the diet may lower fecundity and was found
to be detrimental by interfering with fatty acids essential for normal
wing development in adults. Various modifications in the dietary content
of wheat germ, wheat germ oil or linseed oil resolved the problem
associated with faulty adult emergence. However, there were
variable results because this species must mate within hours of adult
emergence (either sex) for optimum fecundity. The results of nutritional
experiments were used to develop a meridic diet for C. trisectus.
The species was reared successfully for three generations on
a meridic diet with success rates for normal adults of 87%, 82%,
and 72%.
Stock cultures of C. trisectus laboratory reared on Chewings
fescue failed to produce viable eggs after the third generation of
adults. This problem apparently has been overcome by rearing
the species on the meridic diet. No loss of fertility after the third
generation occurred and 56 of 60 larvae in a fourth generation survived
after three weeks.
Genre Thesis/Dissertation
Topic Pyralidae
Identifier http://hdl.handle.net/1957/46046

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