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Polymorphic, sympatric flavonoid variants of Claytonia perfoliata Donn from the Columbia River Gorge

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Title Polymorphic, sympatric flavonoid variants of Claytonia perfoliata Donn from the Columbia River Gorge
Names Miller, John M. (creator)
Chambers, Kenton L. (advisor)
Date Issued 1975-05-12 (iso8601)
Note Graduation date: 1975
Abstract Claytonia perfoliata is a west-American polyploid complex of
common annuals (x=6). The species is polymorphic; exomorphic
characters are often variable within local populations. In a transect
of the Columbia River Gorge diploids, tetraploids, hexaploids, octoploids,
and decaploids were collected, which varied in shape of basal
and cauline leaves, degree of cauline leaf fusion, color of corolla and
calyx, robustness of growth, size of flower parts and seeds, and foliage
coloration. Progeny from field collections were grown in uniform
regimes of light, temperature, and moisture. Each family of progeny
was morphologically indistinguishable from its individual parent implying
a high degree of self-fertilization in nature. The form and sequence of
maturation of basal leaves was found to be an important character differentiating
the families of progeny. Phenolic compounds from
whole plants cultured under uniform greenhouse conditions were extracted (from fresh plant material) in stages using acetone and
methanol, and were separated using two-dimensional paper chromatography.
The flavonoids of petal, sepal, leaf, stem, and whole plant
methanolic extracts (from dried plant material) of wild populations
were separated and isolated. Striking chromatographic differences
between morphological forms, both within and between chromosome
levels were found. Chromatographic spots were identified on the basis
of a set of six spectra, color reactions, and Rf values. Though preliminary
in scope, this study demonstrates the value and reliability
of certain chemical, morphological, and cytological characters as
valuable taxonomic criteria applicable to further study of C. perfoliata.
Genre Thesis/Dissertation
Identifier http://hdl.handle.net/1957/44209

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