Record Details
Fertilization of salmonid eggs with cryopreserved sperm
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Fertilization of salmonid eggs with cryopreserved sperm |
| Names |
Ott, Alvin G.
(creator) Horton, Howard F. (advisor) |
| Date Issued | 1969-09-04 (iso8601) |
| Note | Graduation date: 1970 |
| Abstract | Numerous fishery researchers have attempted to preserve viable fish sperm, but only three investigations were successful in the fertilization of fresh eggs with frozen and thawed sperm: Blaxter (1953); Hoyle and Idler (1968); and Graybill and Horton (1969). Significant advances reported in this thesis were: the development of suitable life extenders for semen of steelhead trout (Salmo gairdneri), and coho (Oncorhynchus kisutch) and Chinook (0. tshawytscha) salmon; the selection of a life protector composed of 8.0% dimethyl. sulfoxide; the verification that the best rate of freezing semen was -30 C/mm, arid that the best method of thawing frozen sperm was in a 4 C water bath; and the determination that no equilibration time was necessary when fresh semen was diluted with extenders and protectors. Application of the above techniques led to the successful cryo-preservation of male gametes of steelhead trout and Chinook and coho salmon in liquid nitrogen (-196 C). Frozen and thawed sperm was mixed with fresh eggs and resulted in maximum fertilizations of 59.1% for steelhead trout, 38.4% for spring Chinook salmon, and 79.2% for coho salmon. Details of the chemical constituents of the extenders and protectors tested are appended. |
| Genre | Thesis/Dissertation |
| Topic | Spermatozoa |
| Identifier | http://hdl.handle.net/1957/22561 |
