Record Details
Field | Value |
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Title | Growth and survival of zebrafish, Brachydanio rerio, larvae fed on two types of microparticulate diets |
Names |
Onal, Umur
(creator) Langdon, Christopher J. (advisor) |
Date Issued | 1997-08-28 (iso8601) |
Note | Graduation date: 1998 |
Abstract | Effects of diet and container type on growth and survival of altricial zebrafish, Brachydanio rerio, larvae were determined. Microfeast® L- 10 (MF) supported growth and survival of zebrafish larvae during the first 10-12 days. Best results were obtained with larvae fed on a combination of MF and Artemia nauplii in Imhoff, 1.2 1 cones. The characteristics of two types of microparticulate diets were determined. MF was encapsulated within cross-linked protein walled capsules (CLPWC) or gelatin-alginate beads (GAB). Retention efficiencies were determined by encapsulating a nontoxic dye (Poly-R 478) within CLPWC or GAB. Retention efficiencies of both particle types suggested that high molecular weight, water soluble nutrients could be delivered to freshwater fish larvae without major losses. Acceptability experiments included determination of gut fullness and feeding incidence of larvae using a computer-aided image analysis system. Acceptability of GAB by first feeding zebrafish larvae was significantly greater than that for CLPWC. Acceptability of CLPWC increased in larger size larvae and was similar to that for GAB. It was determined that both particle types were digested by first feeding zebrafish larvae. Diet size selection of first feeding and 15 day-old zebrafish larvae was determined with CLPWC using a model developed to account for both settlement of capsules and ingestion by larvae. Results indicated that while first feeding larvae preferred 21-45 [micro]m capsules, 15 day-old larvae preferred capsules in the 46-75 [micro]m size range. Growth experiments with CLPWC showed that up to 40% substitution of Artemia nauplii could be accomplished without reduced growth and survival of zebrafish larvae after a feeding period of 8 days. Twenty percent substitution of Artemia nauplii could be achieved with GAB without reduced growth and survival. Higher substitutions of Anemia with either particle types resulted in inferior growth and survival of zebrafish larvae. |
Genre | Thesis/Dissertation |
Topic | Zebra danio -- Growth |
Identifier | http://hdl.handle.net/1957/21883 |