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Growth and survival of zebrafish, Brachydanio rerio, larvae fed on two types of microparticulate diets

ScholarsArchive at Oregon State University

Field Value
Title Growth and survival of zebrafish, Brachydanio rerio, larvae fed on two types of microparticulate diets
Names Onal, Umur (creator)
Langdon, Christopher J. (advisor)
Date Issued 1997-08-28 (iso8601)
Note Graduation date: 1998
Abstract Effects of diet and container type on growth and survival of altricial zebrafish,
Brachydanio rerio, larvae were determined. Microfeast® L- 10 (MF) supported growth
and survival of zebrafish larvae during the first 10-12 days. Best results were obtained
with larvae fed on a combination of MF and Artemia nauplii in Imhoff, 1.2 1 cones.
The characteristics of two types of microparticulate diets were determined. MF
was encapsulated within cross-linked protein walled capsules (CLPWC) or gelatin-alginate
beads (GAB). Retention efficiencies were determined by encapsulating a nontoxic
dye (Poly-R 478) within CLPWC or GAB. Retention efficiencies of both particle
types suggested that high molecular weight, water soluble nutrients could be delivered to
freshwater fish larvae without major losses. Acceptability experiments included
determination of gut fullness and feeding incidence of larvae using a computer-aided
image analysis system. Acceptability of GAB by first feeding zebrafish larvae was
significantly greater than that for CLPWC. Acceptability of CLPWC increased in larger
size larvae and was similar to that for GAB. It was determined that both particle types
were digested by first feeding zebrafish larvae.
Diet size selection of first feeding and 15 day-old zebrafish larvae was
determined with CLPWC using a model developed to account for both settlement of
capsules and ingestion by larvae. Results indicated that while first feeding larvae
preferred 21-45 [micro]m capsules, 15 day-old larvae preferred capsules in the 46-75 [micro]m size
range.
Growth experiments with CLPWC showed that up to 40% substitution of Artemia
nauplii could be accomplished without reduced growth and survival of zebrafish larvae
after a feeding period of 8 days. Twenty percent substitution of Artemia nauplii could be
achieved with GAB without reduced growth and survival. Higher substitutions of
Anemia with either particle types resulted in inferior growth and survival of zebrafish
larvae.
Genre Thesis/Dissertation
Topic Zebra danio -- Growth
Identifier http://hdl.handle.net/1957/21883

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