Record Details
Substrate capture, uptake, and utilization of some amino acids by starved cells of a psychrophilic marine vibrio
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | Substrate capture, uptake, and utilization of some amino acids by starved cells of a psychrophilic marine vibrio |
| Names |
Glick, Melissa Ann
(creator) Morita, Richard Y. (advisor) |
| Date Issued | 1980-08-08 (iso8601) |
| Note | Graduation date: 1981 |
| Abstract | Starved cells of Ant-300, a psychrophilic marine Vibrio, maintained binding and transport systems for glutamic acid, arginine, proline, and leucine. The uptake of these amino acids was inhibited by the uncoupler 2,4-dinitrophenol indicating that an energized membrane was necessary for binding and was maintined in starved cells. The levels of macromolecular synthesis and CO₂ production decreased after the cells had starved for 24 hours and remained low for 30 days of starvation. Most of the amino acid taken up by starved cells was held in shock releasable pools. The substrate affinity of the leucine and arginine binding proteins increased after 48 hours of starvation while the velocity decreased. These starvation induced changes remained constant throughout the 20 day starvation time of the experiment. The maintenance of binding proteins and an energized membrane by a starved cell which has no energy input means that the cell is not committed to total inactivity. The survival form is more of a 'ready and waiting' form, capable of maintaining itself in an unfavorable environment while retaining the ability to take advantage of any favorable shift in its surroundings. |
| Genre | Thesis/Dissertation |
| Topic | Psychrophilic bacteria |
| Identifier | http://hdl.handle.net/1957/30113 |
