Record Details
DAPI direct counting underestimates bacterial abundances and average cell size compared to AO direct counting
ScholarsArchive at Oregon State University
| Field | Value |
|---|---|
| Title | DAPI direct counting underestimates bacterial abundances and average cell size compared to AO direct counting |
| Names |
Suzuki, Marcelino T.
(creator) Sherr, Evelyn (creator) Sherr, Barry F. (creator) |
| Date Issued | 1993 (iso8601) |
| Abstract | We compared estimates of bacterial abundance and of average bacterial cell size based on direct counts of bacterioplankton cells stained with acridine orange (AO) or 4’-6-diamidino-2-phenylidole (DAPI). Estimates of numbers mll–1 of bacteria stained with DAPI were on average 70% (range 57–82%) of bacterial counts made with AO as the stain. Cell volumes estimated from DAPI-stained cells were even less, on average 59% (range 34–80%) of those determined from measurement of AO-stained cells. The combination of lower DAPI values for both cell numbers and cell volumes resulted in overall underestimation of total bacterial biovolume liter–1. DAPI-determined total biovolumes ranged from 25 to 61% of biovolume values obtained via counts and measurements of AO-stained cells in the two data sets. These results can be ascribed to the differential staining of cell components by DAPI and AO. We recommend that DAPI staining be used with caution for estimating bacterial standing stock parameters. |
| Genre | Article |
| Identifier | Limnol. Oceanogr., 38(7), 1993, 1566-1570 |
